RNA-seq results were confirmed by WB analysis of S100A7 in cells harvested from soft and stiff IPNs (Fig. 2m; and Supplementary Fig. 7), IHC of S100A7 in breast cancer patient tissues (Fig. 2n), and qPCR of cells harvested from soft and stiff IPNs (Supplementary Fig. 13). The gene discussed is S100A7; the disease is breast cancer.