ASNS and cancer: Consistent with our previous observation of a dependency between glycolytic flux and intermediates in one-carbon metabolism, SRCAP activity levels across the 1037 CCLE cancer cell lines strongly correlate (R ≥ 0.5) with the expression of three genes (Supplementary Data 2): MTHFD2, a mitochondrial enzymes in folate metabolism, asparagine synthetase (ASNS) and ATF4, an upstream regulator of serine biosynthesis and mitochondrial folate enzyme transcription, involved in the response to amino acids starvation (Supplementary Fig. 6).