Similarly, depletion of ITCH in melanocytes (Supplementary Fig. 4c) and BRAFWT-expressing WM3918 (Fig. 3c), WM1346 (Fig. 3d, e), M245 (Supplementary Fig. 4d), and IPC-298 (Supplementary Fig. 4e) melanoma cells led to a remarkable reduction of p-MEK and p-ERK levels. This evidence concerns the gene ITCH and melanoma.