Double immunolabeling of CD3 and CD68 confirmed results obtained by in situ sequencing, as CD3+ cells located both in 4′,6-diamidino-2-phenylindole (DAPI)-rich and surrounding, DAPI-lighter areas of the lesion, whereas CD68+ located in DAPI-light granuloma areas (Supplementary Fig. 3B). Here, CD68 is linked to Granuloma.