Next, we performed E/N-cadherin single KD or double KD (DKD) in TKO HCC cells and found that silencing of both cadherins leads to reduced half-life and decreased expression of β-catenin (Fig. 5e, f and Supplementary Fig. 5B), without any effect on Wnt target gene (glul, tbx3, and axin2) expression (Supplementary Fig. 5C). This evidence concerns the gene CDH17 and hepatocellular carcinoma.