Analysis of the control liver, and primary and serially transplanted TKO HCC samples indicates that EGFR expression decreases in TKO HCC vs. control livers (likely as a consequence of decreased mRNA abundance; Supplementary Fig. 7B) and its activation (phosphorylation of the Tyr1068 residue as a readout) becomes heterogeneous. Here, EGFR is linked to hepatocellular carcinoma.