FOS and in situ carcinoma: Effects of CIS on uninjured and thermal injured rats PFC and hypothalamic c-Fos levels. c-Fos protein levels in the PFC and hypothalamus are shown by Simple Western blot representative image (above quantification graphs of A–D). CIS exposure had no significant effect on right and left side c-Fos protein expression within and between NS and S groups in the PFC (A) and hypothalamus (B).