Thus, the increased PC (22:6n-3) to total PC ratio (20%, p ≤ 0.05) found in HF–RD rats when compared with HF–SD rats (Figure 3d) suggests decreased PEMT flux in their livers, since previous studies indicated a correlation between the PC–DHA/PC ratio and the estimation of PEMT activity [8,13]. The gene discussed is PEMT; the disease is hydrops fetalis.