In their study, Culp et al. [28] used the B16-F10 melanoma cell line model in which MIF expression was inhibited to demonstrate that MIF produced by melanoma cells acts on angiogenesis by downregulating the expression of the anti-angiogenic factor thrombospondin 1 (TSP-1), which inhibits endothelial cell migration [29]. This evidence concerns the gene THBS1 and melanoma.