After 48 h of transfection, TRPML-1 mRNA levels increased by about 60% (Figure S1c), and after 72 h, protein levels were confirmed higher in pCMV-pTRPML-1 with respect to pCMV T98 and U251 transfected glioma cells (Figure S1d). The gene discussed is MCOLN1; the disease is central nervous system cancer.