Treatment with MK6-83 at 10 μM in T98 and 25 μM in U251 cells induced [Ca2+]i rise in both Ca2+ free medium-treated glioma cell lines with respect to untreated cells, suggesting that the TRPML-1 channel is functional and promotes Ca2+ release from intracellular stores (Figure 4a). The gene discussed is MCOLN1; the disease is central nervous system cancer.