AQP5 and malignant colon neoplasm: AqB011, another bumetanide-derived potent AQP1 ion channel inhibitor with no effect on its water channel activity at up to 200 μM, was shown to impair wound closure of AQP1 expressing HT-29 human colon cancer cell line, however, the finding was not replicated in SW-480, another human colon cancer cell line which expresses AQP5, but not AQP1 [35].