INS and Hypoglycemia: 48) to determine whether repeated insulin-induced hypoglycemia reduced the activation of C1 and C3 medullary neurons. Our specific aims were to determine whether 1) a 3-day repeated hypoglycemia protocol reduces C1 and C3 neuronal activation and 2) a hypoglycemia prevention protocol or 3) injection of naloxone during antecedent hypoglycemia in HAAF rats can restore C1 and C3 neuronal activation and epinephrine release after subsequent hypoglycemia.