After a 4 hr infection with L. interrogans strain Lai and removal of extracellular leptospires during a 24 hr subsequent incubation (re-incubation), the intracellular leptospires were released from all the infected cells, while the leptospiral release were prevented by Rab11, Sec15, Sec3, VAMP2 or SYN1 depletion, LF + PA, EF + PA, BoNT/C-LC or BoNT/D-LC treatment, or MF, MT or FAK but not PI3K inhibition (Figure 6A). Here, SYN1 is linked to infection.