To detect the effect of RhoB on PTEN/AKT signaling pathway in breast cancer cells, we respectively used Western blot to detect changes in the expression levels of PTEN/AKT signaling pathway-related protein AKT, p-AKT, and PTEN after overexpression and knockdown of RhoB in MDA-MB-231 cells and MCF-7. Here, RHOB is linked to breast cancer.