In addition to the overlap between fitness factors for bacteremia and UTI/CAUTI, seven of the 143 infection-specific fitness factors identified in this screen were previously verified as contributing to secondary bacteremia during co-challenge with the WT strain: gdhA, pta, and cysJ for ascending UTI [29, 54, 55], and arnA, glnA, lon, and argR for CAUTI [28]. Here, LONP1 is linked to bacterial urinary tract infection.