To test the impact of the probiotic, prebiotic and their combination on the survivability of both wildtype and AD Drosophila, 5 independent groups of 10 male Drosophila were isolated after eclosion from each treatment group either with the elav-Gal4 only genetic background (wildtype) or the crossed APP-BACE1 AD model. Here, APP is linked to Alzheimer disease.