To examine whether downregulation of CD164 in GBM cells had an impact on these events, we examined senescence-associated β-galactosidase (SA-β gal) staining to assess cellular senescence, DCFH-DA fluorescence as a surrogate for reactive oxygen species (ROS) generation associated to oxidative stress, and caspase 3/9 and LC3B expression to assess apoptosis and autophagy respectively. The gene discussed is CASP3; the disease is glioblastoma.