In this study, compared with other E3 ubiquitin ligases, the decrease in ARHGAP26 protein expression in cells with SMURF1 overexpression was the most significant and SMURF1 overexpression promoted ARHGAP26 ubiquitination and ovarian cancer cell invasion and migration, along with an increase in β-catenin, VEGF, MMP2, and MMP7 expression. The gene discussed is MMP2; the disease is ovarian cancer.