Studies evaluating the organization and expression of intermediate filaments in glioblastoma found the following three distinct subpopulations with: (1) High vimentin, GFAP, and synemin expression or (2) low vimentin, GFAP, and synemin expression or (3) high nestin but low vimentin, GFAP, and synemin expression [533]. This evidence concerns the gene NES and glioblastoma.