As a precautionary note, although the Nuc+Cyt subtype is progressively decreased in ECA/GEJ Aca progression, the evidence that both the Cyt subtype and the Nuc+Cyt subtype persisted in the full spectrum of ECA/GEJ Aca differentiation grade underscores the need for additional molecular markers that may serve as cancer risk factors within the Nuc+Cyt subtype. The gene discussed is NUCB1; the disease is cancer.