Binding studies using flow cytometry as the readout demonstrated that the ~55KDa CD3xPDL1 BiTE binds to CD3+ human peripheral blood mononuclear cells (PBMC), and to PD-L1+ human melanoma, chronic myelogenous leukemia, and lung adenocarcinoma cell lines, but not to CD3− or PD-L1− human tumor cells. Here, CD274 is linked to melanoma.