To further assess whether JMJD3 and p300 are required for NOTCH genes transcriptional activation and to study their functional role in Notch-dependent T-ALL cell contexts, we evaluated the effects of the inhibition of their enzymatic activities on the expression of NOTCH3, NOTCH1 and most prominent Notch target genes and on the proliferation rate in different T-ALL cell lines. Here, NOTCH3 is linked to acute lymphoblastic leukemia.