LGR5 and colorectal cancer: One of the difficulties of research on stem cell markers is that the evaluation method varies widely for different studies and the results obtained can be inconsistent.11 We used 5% as a cut-off level for LGR5 based on a previous study on colorectal cancer using the same antibody.13 In order to ensure maximum credibility of the results, we used pathological sections which included not only the invasive front but also the normal colon tissue whenever possible, so that the normal part can be used as an internal control.