ISM1 and adrenal gland neoplasm: Several genes previously found overexpressed in other (non-APA) types of CTNNB1-mutated adrenal tumours were overexpressed in CTNNB1-mutated APAs: AFF3, ISM1, NKD1, ENC1 and RALBP1. AFF3 and ISM1 were selected for validation by RT-qPCR, and their overexpression confirmed (Fig. 1d,e).