To further confirm all the above in vitro data showing that the pseudogene ASS1P3 might function as a ceRNA mediated by AR to suppress cell proliferation by sponging miR-34a-5p in RCC, we generated four groups of OSRC-2-Luc cells, also transfected with (1) pWPI + pLVTHM, (2) oeAR + pLVTHM, (3) pWPI + oeASS1P3, and (4) oeAR + oeASS1P3, and inoculated these cells (1 × 106) into the left renal capsules of nude mice. This evidence concerns the gene AR and renal cell carcinoma.