Analysis of TCGA database with more than 600 human RCC samples also revealed that patients with a lower ASS1 expression had a significantly lower disease-free survival (HR = 1.432, P = 0.0059) as well as overall survival (HR = 1.498, P = 0.0054) than those with a higher ASS1 expression (Fig. 2f, g), respectively. The gene discussed is ASS1; the disease is renal cell carcinoma.