As expected, overexpression of EGOT increased LC3-II/LC3-I levels and reduced P62 expression in HeLa and breast cancer cells (Fig. 3a and b; Additional file 2: Figure S3f and g), while knockdown of EGOT reduced LC3-II/LC3-I levels and increased P62 expression (Fig. 3c and d). This evidence concerns the gene SQSTM1 and breast cancer.