By employing an unbiased ubiquitin proteomic approach, further studies demonstrated that Rngo was a target of the E3 ligase UBE3A [53] and, interestingly, UBE3A-mediated Rngo ubiquitylation was shown to be conserved in SH-SY5Y neuroblastoma cells in which the human UBE3A homolog also has the ability to target and ubiquitylate Ddi1 [53]. This evidence concerns the gene UBE3A and neuroblastoma.