Given the stark baseline reduction in small intestinal Foxp3+ Tregs in Itgb8 (CD11c-Cre) mice (Fig 2E), we utilised the DEREG mouse model, which allows specific ablation of Foxp3+ Tregs by injection of diphtheria toxin [32], to directly test the functional role of Foxp3+ Tregs during infection. This evidence concerns the gene FOXP3 and infection.