All of the results mentioned showed that KCNQ1OT1 overexpression reduced the percentage of MTX‐resistant CRC cells arrested in G0 phase and suppressed cell apoptosis, whereas the silencing of KCNQ1OT1 induced G0/G1 cell cycle arrest in MTX‐resistant CRC cells and accelerated cell apoptosis. Here, KCNQ1OT1 is linked to colorectal carcinoma.