Towards controlling the increasing problem of leptospirosis, for the first time we evaluated, LenA and LcpA proteins from L. interrogans serovar Copenhageni strain Fiocruz L1-130 in combination with E. coli heat-labile enterotoxin, B subunit (LTB) as potential candidates for vaccine production against Leptospiral infections. Here, LTB is linked to leptospirosis.