Since MVP predominantly localized in macrophages in obese adipose tissues, we further generated a mouse model with myeloid-specific deletion of MVP (MacKO, MVPflox/floxLyz2-Cre) by establishing MVPflox/flox mice that were crossed with Lyz2-Cre mice (Supplementary Fig. 4a-b) to investigate the role of macrophage MVP in the pathogenesis of metabolic disorders. This evidence concerns the gene MVP and metabolic disease.