Ferroptosis is a metabolic disorder of lipid peroxidation; therefore, lipid peroxides cannot undergo GSH metabolism catalysed by GPX4, which produces a large amount of toxic lipid ROS.18, 31 To explore the level of ferroptosis and ALR expression in HK‐2 cells after I/R treatment, the expression of acyl‐CoA synthetase long‐chain family member 4 (ACSL4), transferrin receptor (TFR), SLC7A11, GPX4 and ALR was determined by Western blotting (Figure 1A). Here, GPX4 is linked to metabolic disease.