Given the complex relationship between Kiss1/kisspeptin/GPR54 pathway and HPG axis, we aimed to identify the polymorphisms in Kiss1 and GPR54 genes and investigate the association between Kiss1 and GPR54 gene polymorphisms and serum kisspeptin levels in normal Sri Lankan women and those manifesting well characterized PCOS from adolescence. This evidence concerns the gene KISS1 and polycystic ovary syndrome.