The experiment shown in Fig. 2A–C demonstrates that the reduction in tumor growth at day 25 for the combination treatment (257.3 mm3) was significantly smaller than those mice that were treated with either NextA (750.1 mm3) or anti-PD-1 (1030 mm3) in comparison with the control group (2034 mm3). This evidence concerns the gene PDCD1 and neoplasm.