Further, treatment of GBM cells with TMZ (for 48 h and 72 h) resulted in elevated levels of γH2AX foci compared to control cells; this effect was significantly enhanced in FlagERβ expressing U251 cells (Fig. 5A,B) Collectively, these results suggests that ERβ enhances the DNA damage induced by TMZ treatment. Here, ESR2 is linked to glioblastoma.