Interestingly, both clones changed marker expression in vivo, i.e., pseudodiploid clones retained a heterogeneous A2B5 profile and increased CD133, while aneuploid clones significantly increased A2B5 epitope presentation (Fig. 1d), indicating that CSC-associated marker expression in vivo is not a defined property of a genetic clone and that tumor cells change marker expression following clonal selection, most probably reflecting an adaptation to the new microenvironment. This evidence concerns the gene PROM1 and neoplasm.