To analyze the inflammatory status of the lungs during infection and after fat ablation, we performed qPCR analysis and measured the levels of various proinflammatory (IL-6, TNF-α, IFN-γ, CD68, and IL-12) and anti-inflammatory (IL-10) cytokines, and the mRNA levels of M2 macrophage markers (Arg1 and FizzI) (Fig. 5a). This evidence concerns the gene TNF and infection.