To determine whether the expression of oncogenic PIK3CA mutants increases RNMT dependency in additional cell lines, PIK3CA WT, C420R, E545K, H1047R and vector control were expressed in the non-transformed mammary epithelial cell line IMEC and the breast cancer cell line MDA-MB-231 (figure 5a,e). This evidence concerns the gene PIK3CA and breast carcinoma.