To examine the role of NLRP12 in the regulation of JNK in non-parenchymal cells, such as Kupffer cells and hepatic stellate cells, in the tumor microenvironment, we isolated hepatocytes, Kupffer cells, and hepatic stellate cells from WT and Nlrp12-/- HCC tissues. Here, NLRP12 is linked to hepatocellular carcinoma.