To examine whether tissue of origin influences the metabolic makeup of the tumor microenvironment, cancer cells derived from lung (Jackson et al., 2005; Jackson et al., 2001) and PDAC (Bardeesy et al., 2006) tumors both driven by activation of Kras and loss of Trp53 were injected subcutaneously into C57BL/6J mice, such that tumors were established in the same location and with the same oncogenic driver mutations, but different tissues of origin (Figure 5A). This evidence concerns the gene TP53 and neoplasm.