Double Annexin V/propidium iodide staining followed by flow cytometry revealed that 0.4 μM AG increased the frequency of Annexin V-positive cells in a time-dependent manner (Fig. 2b), whereas AG at 0.1 μM did not consistently induce apoptosis (Fig. 2b) indicating that AG at this concentration could rather exhibit cytostatic effect in melanoma cell lines (Fig. 2a). Here, ANXA5 is linked to melanoma.