Based on in-depth bioinformatic analysis, the four up-regulated proteins [PTMA, PAK2, PPP1CA, HMGB2) and the five down-regulated proteins Caveolin, Integrin beta-1, Collagen alpha-2(VI), Leiomodin-1 and Vinculin] were selected and validated in ESCC by Western Blot. This evidence concerns the gene HMGB2 and esophageal squamous cell carcinoma.