In summary, the co-enrichment of ESCRT-III and RPA/cGAS at a subset of micronuclei and the absence of RPA/cGAS enrichment upon CHMP7 depletion, point to a non-canonical (VPS4-independent) function for ESCRT-III in maintaining a pool of cytosolic DNA in cancer cells. The gene discussed is VPS4A; the disease is cancer.