VPS4A and cancer: Our VPS4KD data imply the existence of a similar mechanism; hence, the accumulation of CHMP7 and CHMP4B at micronuclei observed in unperturbed cancer cells implies the normal balance between ESCRT-III assembly and disassembly is impaired and/or is not regulated by VPS4 (VPS4-independent pathway, Supplemental Fig. 4B).