Berberine induced a decrease in activity of two DNA methylases, DNMT1 (DNA (cytosine-5)-methyltransferase 1) and DNMT3, that, through DNA hypomethylation, induced an increase of p53 [60]; p53 activation was observed following the increase in miR-23a, through repression of Nek6, in HCC cells in response to Rhizoma Coptis aqueous extract [60]. Here, TP53 is linked to hepatocellular carcinoma.