In support of this data in clinical samples, immunoblotting analysis revealed that RORα1 expression dramatically decreased in the prostate metastatic cancer cell line such as LNCaP compared to that in the normal prostate cell line such as RWPE1, while the expression of c-jun increased in LNCaP cells and decreased in RWPE1 cells (Figure 4C). This evidence concerns the gene JUN and metastatic malignant neoplasm.