This research has referred to the predecessor in the experiment design regarding timing, such as the p-ERK activation being observed until 24 h after stimulation in breast cancer [35], NE-like LNCaP cells [36], and GC-2 cells [37], and the phosphorylated protein kinase C (p-PKC) activation at 24 h was also observed in ovarian surface epithelial cancer cells [38], GC-2 cells [37], podocytes [39], glial cells [40], and HT22 cells [41]. The gene discussed is PRRT2; the disease is breast carcinoma.