In C57BL/6 mice, β-sitosterol (20 mg/kg/day) administration for 56 days prevented the colon shortening (~8%) and reduced MPO activity in colon tissue (~35%), what led to a lower level of pro-inflammatory cytokines (IL-1β, TNF-α and IL-6) after colitis induction by high fat diet (60 Kcal% from fat) [16]. The gene discussed is TNF; the disease is colitis.