In addition, real-time PCR and western blot analyses of the high grade urothelial carcinoma cell lines (J82, BFTC909, and T24) displayed lower SPARCL1 expression levels than the low grade urothelial carcinoma cell line (RT4) and the immortalized human urothelial cells (SV-HUC-1) in Figure 3E,F. Here, SPARCL1 is linked to urothelial carcinoma.