Our experimental results indicated that AICAR suppresses TGF-β-induced cell migration and invasion as well as inhibits TGF-β-induced EMT by decreasing the expression of a mesenchymal-related marker, N-cadherin and enhancing the expression of an epithelial-related marker, E-cadherin in 22Rv1 prostate cancer cells. This evidence concerns the gene CDH2 and Familial prostate cancer.