The rs738409 in the patatin like phospholipase domain containing 3 (PNPLA3) gene encoding for an isoleucine to methionine substitution at position 148 (PNPLA3 I148M) of the protein is the strongest genetic variation increasing the risk of NAFLD [23]. This evidence concerns the gene PNPLA3 and metabolic dysfunction-associated steatotic liver disease.