For NSCLC, %TCmRNA threshold at 18% and dots/tumour cell cut off at 0.26, 85.7% of PD-L1 high IHC cases would also be PD-L1 high by RNAScope and 78.6% of PD-L1 low IHC cases would also be classified as PD-L1 low by RNAScope. This evidence concerns the gene CD274 and neoplasm.